Trail Location: South
- A01. Quick Point Nature Preserve
- A02. Arlington Park
- A03. Pinecraft Park
- A04. Celery Fields
- A05. Crowley Museum and Nature Center
- A06. Myakka River State Park
- A07. Red Bug Slough Preserve
- A08. Siesta Beach
- A09. Oscar Scherer State Park
- A10. T. Mabry Carlton, Jr. Memorial Reserve
- A11. Jelks Preserve
- B12. Venice Area Audubon Rookery
- B13. Shamrock Park and Nature Center
- B14. Caspersen Beach
- B15. Lemon Bay Park and Environmental Center
- B16. Blind Pass Beach Park
- B17. Indian Mound Park
- B18. Charlotte Harbor Environmental Center: Cedar Point Environmental Park
- B19. Amberjack Environmental Park
- B20. Tippecanoe Environmental Park
- C21. Charlotte Harbor Environmental Center: Alligator Creek Preserve
- C22. Charlotte Harbor Preserve State Park: Old Datsun Trail
- C23. Fred C. Babcock/Cecil M. Webb Wildlife Management Area
- C24. Charlotte Flatwoods Environmental Park
- C25. Charlotte Harbor Preserve State Park: North Cape Flats Trail
- D26. Charlotte Harbor Preserve State Park: Little Pine Island Trail
- D27. Cayo Costa State Park
- D28. J. N. “Ding” Darling National Wildlife Refuge
- D29. Lighthouse Park Beach
- D30. Rotary Park Environmental Center
- D31. Lakes Regional Park
- D32. San Carlos Bay: Bunche Beach Preserve
- D33. Bowditch Point Regional Park
- D34. Matanzas Pass Preserve
- D35. Little Estero Island Critical Wildlife Area
- E36. Lovers Key State Park
- E37. Estero Bay Preserve State Park
- E38. Six Mile Cypress Slough Preserve
- E39. Hickey’s Creek Mitigation Park
- E40. Caloosahatchee Regional Park
- E41. CREW: Corkscrew Marsh Unit
- E42. Corkscrew Swamp Sanctuary (Gateway)
- F43. Fisheating Creek Wildlife Management Area: East Entrance
- F44. Fisheating Creek Wildlife Management Area: West Entrance
- F45. Okaloacoochee Slough State Forest and Wildlife Management Area
- F46. Dinner Island Ranch Wildlife Management Area
- F47. Stormwater Treatment Area 5
- G48. Stormwater Treatment Area 1 West
- G49. Royal Palm Beach Pines Natural Area
- G50. Grassy Waters Preserve (City of West Palm Beach)
